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Basler
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Image Search Results
Journal: Biomolecules & Therapeutics
Article Title: Late Passage Cultivation Induces Aged Astrocyte Phenotypes in Rat Primary Cultured Cells
doi: 10.4062/biomolther.2020.175
Figure Lengend Snippet: Late passage astrocytes show reduced mitochondrial membrane potential and ATP production. (A) Astrocytes were conducted for mitochondrial membrane potential by fluorescence staining of TMRM. Representative images were obtained by the IncuCyte ZOOM microscope software 2015A (Essen Bioscience). The data were measured by IncuCyte software (Essen Bioscience). The representative graph shows the total TMRM object integrated intensity (RCU x μm 2 /image) and the total TMRM object area (μm 2 /image). Bars represent the mean ± SEM (n=3). **indicates p <0.01, and ***indicates p <0.001 vs. vehicle group. (B) The mitochondrial OCR of astrocytes was experimented using Cell Mito Test Kit and Agilent Seahorse XFe96 Analyzer (Agilent Technologies). The representative graphs were visualized using Wave Desktop 2.6 software (Agilent Technologies). The graphs represent the basal level, spare respiratory capacity, proton leak, and ATP production level. OCRs are mean ± SEM (n=3), normalized to the third measurement point and expressed as % baseline OCR. *indicates p <0.05 and, ***indicates p <0.001 vs. P1 astrocytes.
Article Snippet: We detected the red fluorescence using the IncuCyte ZOOM system (Essen Bioscience) by imaging each well every 30 min for 12 h and analyzed the red particles using the
Techniques: Membrane, Fluorescence, Staining, Microscopy, Software